王毅芳;杨志辉;朱杰华;张岱;赵冬梅;张健;

• 1:河北农业大学植物保护学院

摘要(Abstract)：

尖镰孢寄主范围广、遗传差异大,其种下存在多种寄主专化型。对尖镰孢寄主专化型的快速鉴定可为科学制定植物病害防控策略提供依据。利用Sherlock MIS脂肪酸鉴定系统对分离自番茄、棉花、黄瓜、茄子等4种寄主专化型的18株尖镰孢进行脂肪酸成分测定,共检测到10种脂肪酸。运用SPSS软件中的PCA法对被检测到的脂肪酸进行主成分分析,确定了18:1CIS9(W9)[X1],18:2 CIS 9,12/18:0a[X2]和18:00[X3]等3个脂肪酸为其主成分。利用Bayes逐步判别法建立了尖镰孢4种不同寄主专化型判别模型为Y1=‐157.750+2.809X1+3.391X2+8.099X3;Y2=‐178.343+0.586X1+7.587X2‐0.214X3;Y3=‐129.132+2.749X1+4.163X2+4.476X3;Y4=‐201.307+2.016X1+7.345X2+2.400X3。通过对43株未知寄主专化型菌株主成分脂肪酸的测定,利用判别法对尖镰孢进行判定,结果发现有40株与原寄主来源一致,判对率达93%。表明脂肪酸生物标记法可用于尖镰孢寄主专化型的快速鉴定。

关键词(KeyWords)： 尖镰孢;脂肪酸标记;寄主专化型;判别模型

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项资金(CARS-10-P12);; 公益性行业(农业)科研专项(201303018)~~

作者(Authors): 王毅芳;杨志辉;朱杰华;张岱;赵冬梅;张健;

DOI: 10.13346/j.mycosystema.140283

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