设施化专用香菇种质资源挖掘Excavation of special germplasm resources for facility cultivation of Lentinula edodes
张丹;庄振;季英磊;尚晓冬;张美彦;章炉军;
摘要(Abstract):
以香菇L808菌株基因组序列为参照,开发了300份插入/缺失(InDel)标记。通过5个菌株的初筛,选出均匀分布于基因组的82份多态标记对42份香菇种质资源进行遗传背景分析。同时在设施化栽培条件下考察了种质资源的生育期、菌棒转色、菌棒硬度、现蕾期和产量等表型。结果表明:供试香菇种质资源的遗传多样性丰富,栽培菌株与野生菌株的遗传距离较远,遗传相似性系数平均值为0.51。群体结构分析将种质资源分为6个亚群,与基于遗传距离的系统聚类结果较为一致。亚群间菌株具有较远的亲缘关系,遗传分化指数平均值为0.290,适合用于杂交育种。表型结果显示,设施化栽培条件下的种质资源农艺性状分化程度高,亚群间菌株的生育期、现蕾期和产量均有显著差异。种质资源多样性分析结果为香菇杂交育种工作奠定了基础。
关键词(KeyWords): 香菇;InDel标记;遗传多样性;表型;杂交育种
基金项目(Foundation): 上海市现代农业产业技术体系(沪农科产字2022第9号)~~
作者(Authors): 张丹;庄振;季英磊;尚晓冬;张美彦;章炉军;
DOI: 10.13346/j.mycosystema.220135
参考文献(References):
- Adedze YMN,Lu X,Xia YC,Sun QY,Nchongboh CG,Alam MA,Liu MH,Yang X,Zhang WT,Deng ZJ,Li WH,Si LT,2021.Agarose-resolvable In Del markers based on whole genome re-sequencing in cucumber.Scientific Reports,11(1):3872
- Bao DP,2020.Scientific problems in crossbreeding of edible fungi.Acta Edulis Fungi,27(4):1-24 (in Chinese)
- Chang ST,1980.Mushrooms as human food.Bioscience,6(30):399-401
- Dai YC,Yang ZL,2008.A revised checklist of medicinal fungi in China.Mycosystema,27(6):801-824 (in Chinese)
- Danecek P,Auton A,Abecasis G,Albers CA,Banks E,De Pristo MA,Handsaker RE,Lunter G,Marth GT,Sherry ST,Mc Vean G,Durbin R,2011.The variant call format and VCFtools.Bioinformatics,27(15):2156-2158
- Dong H,Shang XD,Cao H,Zhang XX,Liu RN,2012.Correlation analysis between fruiting traits and yield of Lentinula edodes strains.Journal of Southern Agriculture,43(6):810-813 (in Chinese)
- Dong H,Zhang LJ,Zhang MY,Jiang N,Yu HL,Song CY,Shang XD,Tan Q,2017.Genetic diversity and fingerprint profiles of Chinese major Lentinula edodes cultivars based on SSR markers.Microbiology China,44(6):1427-1436 (in Chinese)
- Elena VM,Talía H,Octavio PL,2015.Edible mushrooms:improving human health and promoting quality life.International Journal of Microbiology,20(1):376-387
- Gong WB,Xu R,Xiao Y,Zhou Y,Bian YB,2014.Phenotypic evaluation and analysis of important agronomic traits in the hybrid and natural populations of Lentinula edodes.Scientia Horticulturae,179:271-276
- Huang NL,1994.Lentinula edodes cultivation in China.Shanghai Scientific and Technical Literature Publishers,Shanghai.74-75 (in Chinese)
- Jain A,Roorkiwal M,Kale S,Garg V,Yadala R,Varshney RK,2019.In Del markers:an extended marker resource for molecular breeding in chickpea.PLo S One,14(3):e0213999
- Kuhad RC,Kapoor RK,Lal R,2004.Improving the yield and quality of DNA isolated from white-rot fungi.Folia Microbiologica,49(2):112-116
- Lee HY,Moon S,Ro HS,Chung JW,Ryu H,2020.Analysis of genetic diversity and population structure of wild strains and cultivars using genomic SSR markers in Lentinula edodes.Mycobiology,48(2):115-121
- Li C,Gong WB,Zhang L,Yang ZQ,Nong WY,Bian YB,Kwan HS,Cheung MK,Xiao Y,2017.Association mapping reveals genetic loci associated with important agronomic traits in Lentinula edodes,Shiitake mushroom.Frontiers in Microbiology,8:237
- Li Y,Li TH,Yang ZL,Bau T,Dai YC,2015.Atlas of Chinese macrofungal resources.Central China Farmer Press,Zhengzhou.1-1351 (in Chinese)
- Lin FC,1995.Genetic correlations and path coefficient analysis of some important quantitative characteristics in Lentinula edodes.Acta Edulis Fungi,2(2):9-12 (in Chinese)
- Liu J,Wang ZR,Li C,Bian YB,Xiao Y,2015.Evaluating genetic diversity and constructing core collections of Chinese Lentinula edodes cultivars using ISSR and SRAP markers.Journal of Basic Microbiology,55(6):749-760
- Liu JY,Ying ZH,Liu F,Liu XR,Xie BG,2012.Evaluation of the use of SCAR markers for screening genetic diversity of Lentinula edodes strains.Current Microbiology,64(4):317-325
- Liu K,Muse SV,2005.Power marker:an integrated analysis environment for genetic marker analysis.Bioinformatics,21(9):2128-2129
- LüZZ,Liu QY,1996.Progress and prospects of shiitake breeding on China.Edible Fungi of China,15(1):5-7 (in Chinese)
- Moon S,Lee HY,Shim D,Kim M,Ka KH,Ryoo R,Ko HG,Koo CD,Chung JW,Ryu H,2017.Development and molecular characterization of novel polymorphic genomic DNA SSR markers in Lentinula edodes.Mycobiology,45(2):105-109
- Mukhopadhyay K,Haque I,Bandopadhyay R,Covert S,Porter D,2012.AFLP based assessment of genetic relationships among shiitake (Lentinula ssp.) mushrooms.Molecular Biology Reports,39(5):6059-6065
- Pan YJ,Tan Q,Chen MJ,Feng ZY,Wang ZY,1998.The present situation and development of genetic research and spawn selection of Lentinula edodes.Acta Edulis Fungi,5(3):61-66 (in Chinese)
- Shen XF,Zhang LJ,Zhang MY,Shang XD,Li Y,Yu HL,2020.In Del marker and its application and prospection in research of edible fungi.Acta Agriculturae Shanghai,36(3):136-140 (in Chinese)
- Shen XF,Zhang LJ,Zhang MY,Zhang D,Yu HL,Li Y,2021.Genetic diversity and population structure of Lentinula edodes analyzed by In Del markers.Mycosystema,40(9):2266-2281 (in Chinese)
- Song XX,Li CH,Tan Q,Li QZ,Ma DD,Chen MJ,2015.The summary of resources of some cultivated strains of Lentinula edodes in China.Journal of Fungal Research,13(3):146-154 (in Chinese)
- Tan Q,Pan YJ,Huang WY,2000.The development process of Lentinula edodes breeding in China.Acta Edulis Fungi,7(4):48-52 (in Chinese)
- Tian MY,Gao Y,Du ST,Cheng SS,Chen XW,Yang LL,2019.Research on the agronomic traits of 27 Lentinula edodes strains.Journal of China Agricultural University,24(1):82-91 (in Chinese)
- Weir BS,Cockerham CC,1984.Estimating F-Statistics for the analysis of population structure.Evolution,38(6):1358-1370
- Wu F,Zhou LW,Yang ZL,Bau T,Li TH,Dai YC,2019.Resource diversity of Chinese macrofungi:edible,medicinal and poisonous species.Fungal Diversity,98:1-76
- Wu XQ,Li HB,Zhao WW,Fu LZ,Peng HZ,He L,Cheng JW,Wei HL,Wu QQ,2010.SCAR makers and multiplex PCR-based rapid molecular typing of Lentinula edodes strains.Current Microbiology,61(5):381-389
- Xiang XJ,Li C,Li L,Bian YB,Kwan HS,Nong WY,Cheung MK,Xiao Y,2016.Genetic diversity and population structure of Chinese Lentinula edodes revealed by In Del and SSR markers.Mycological Progress,15(4):1-13
- Xiao Y,Liu W,Lu YY,Gong WB,Bian YB,2010.Applying target region amplification polymorphism markers for analyzing genetic diversity of Lentinula edodes in China.Journal of Basic Microbiology,50(5):475-483
- Xu JP,Wang HC,Hu DM,2018.Exploring genotypeenvironment interactions for breeding edible mushrooms.Acta Edulis Fungi,25(2):11-22
- Xu XF,Yang JG,Ning ZX,Zhang XW,2015.Lentinula edodes-derived polysaccharide rejuvenates mice in terms of immune responses and gut microbiota.Food&Function,6(8):2653-2663
- Zhang D,Song CY,Zhang LJ,Wu P,Bao DP,Shang XD,Tan Q,2014.Genetic diversity and fingerprint profiles of commercial Lentinula edodes cultivars based on SSRmarkers developed from the whole genome sequence.Acta Edulis Fungi,21(2):1-8 (in Chinese)
- Zhang RY,Huang CY,Zheng SY,Zhang JX,Ng TB,Jiang RB,Zuo XM,Wang HX,2007.Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers.Applied Microbiology and Biotechnology,74(1):140-145
- 鲍大鹏,2020.食用菌杂交育种中的科学问题.食用菌学报,27(4):1-24
- 戴玉成,杨祝良,2008.中国药用真菌名录及部分名称的修订.菌物学报,27(6):801-824
- 董辉,尚晓冬,曹晖,张旬旬,刘瑞娜,2012.香菇菌株出菇性状与产量的相关分析.南方农业学报,43(6):810-813
- 董慧,章炉军,张美彦,姜宁,于海龙,宋春艳,尚晓冬,谭琦,2017.中国香菇主栽品种遗传多样性的SSR分析及指纹图谱构建.微生物学通报,44(6):1427-1436
- 黄年来,1994.中国香菇栽培学.上海:上海科学技术文献出版社.74-75
- 李玉,李泰辉,杨祝良,图力古尔,戴玉成,2015.中国大型菌物资源图鉴.郑州:中原农民出版社.1-1351
- 林芳灿,1995.香菇主要数量性状的遗传相关及通径分析.食用菌学报,2(2):9-12
- 吕作舟,刘巧云,1996.我国香菇育种的回顾与展望.中国食用菌,15(1):5-7
- 潘迎捷,谭琦,陈明杰,冯志勇,汪昭月,1998.香菇遗传研究和菌种选育的现状及发展.食用菌学报,5(3):61-66
- 沈秀芬,章炉军,张美彦,尚晓冬,李玉,于海龙,2020.In Del标记及其在食用菌研究中的应用与展望.上海农业学报,36(3):136-140
- 沈秀芬,章炉军,张美彦,张丹,于海龙,李玉,2021.利用In Del标记分析中国香菇菌株的遗传多样性与群体结构.菌物学报,40(9):2266-2281
- 宋晓霞,李传华,谭琦,李巧珍,马丹丹,陈明杰,2015.中国香菇部分栽培菌株来源汇总.菌物研究,13(3):146-154
- 谭琦,潘迎捷,黄为一,2000.中国香菇育种的发展历程.食用菌学报,7(4):48-52
- 田梦媛,高原,杜双田,程爽爽,陈晓文,杨丽莉,2019.27个香菇菌株农艺性状比较研究.中国农业大学学报,24(1):82-91
- 张丹,宋春艳,章炉军,巫萍,鲍大鹏,尚晓冬,谭琦,2014.基于全基因组序列的香菇商业菌种SSR遗传多样性分析及多位点指纹图谱构建的研究.食用菌学报,21(2):1-8